Top : Methods and Protocols : Biochemistry
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Bart's Cookbook and Lab Protocols
- This page details the protocols used in the Sefton Lab at the Salk Institute. Among the techniques described are Westerns, ECL, phosphoaminoacid analysis, two-dimensional peptide mapping, CNBr mapping, V8 partial proteolytic mapping, general tissue culture methods and infection with retroviruses, biosynthetic labeling, and immunoprecipitation. The protocols include solution recipes and are filled with useful and practical hints.
http://pingu.salk.edu/users/Sefton_web/Hyper_protocols/TableOfContents.html
Added: 27-Mar-2000 Hits: 4313 Rating: 8.33 Votes: 3
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Biochemical techniques
- List of biochemical techniques from the Melbourne Signal Transduction Group
http://grimwade.biochem.unimelb.edu.au/bowtell/biochem/biochem.htm
Added: 2-Oct-1999 Hits: 5007 Rating: 4.00 Votes: 8
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* Western blot protocol
- This protocol is a gift from Richard Huganir's lab.
http://biophysics.med.jhmi.edu/wolberger/western_blot.html
Added: 17-Apr-2002 Hits: 2379 Rating: 1.50 Votes: 2
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Ambros Lab Protocol Collection
- A comprehensive collection of nematode-related protocols and information, this site includes general methods, equipment, microinjection, staining, dauer protocols, mutants, and drugs. This is a valuable resource for anyone working in this area.
http://www.dartmouth.edu/artsci/bio/ambros/protocols/worm_protocols.html
Added: 20-Apr-2000 Hits: 1746 Rating: 0 Votes: 0
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mRNA Purification
- Protocol for the purification of mRNA.
http://www.fhcrc.org/labs/breeden/Methods/mRNA_purification.html
Added: 23-May-2000 Hits: 1511 Rating: 10.00 Votes: 1
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Purification of mitochondrial DNA
- Purification of mitochondrial DNA: MtDNA from Protists and MtDNA from Fungi.
http://megasun.bch.umontreal.ca/People/lang/FMGP/methods/mtDNA.html
Added: 1-Oct-1999 Hits: 1413 Rating: 0 Votes: 0
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SDS - Polyacrylamide Gels
- Protocols for the preparation of SDS - Polyacrylamide Gels.
http://www.fhcrc.org/labs/breeden/Methods/SDS-PAGE.html
Added: 23-May-2000 Hits: 2200 Rating: 4.75 Votes: 4
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SDS-PAGE
- SDS polyacrylamide gel electrophoresis manual
http://www.uct.ac.za/microbiology/sdspage.htm
Added: 1-Oct-1999 Hits: 2112 Rating: 10.00 Votes: 2
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SDS-PAGE
- Silver Staining of SDS-PAGE gels.
http://www.nwfsc.noaa.gov/protocols/silverstain.html
Added: 21-Mar-2000 Hits: 1542 Rating: 6.00 Votes: 2
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Silver staining of protein gels
- This protocol is adapted from Shevchenko et al., Analytical chemistry 68, p 850-858 (1996) and is especially suited for 1 mm gels.
http://www.fhcrc.org/labs/breeden/Methods/silverstain.html
Added: 23-May-2000 Hits: 1662 Rating: 5.00 Votes: 1
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