Protocol for intracytoplasmic staining of cytokines for FACS analysis.
Quick and simple method for conjugating mAbs.
This note is not intended as a full protocol for making monoclonal antibodies, but is to give a few hints and tips for the use of Y3/Ag1.2.3. Generally speaking the methodology is similar to making murine (eg. with NS0) monoclonal antibodies, but there are a few critical differences
The following method is recommended for purification of monoclonal antibodies produced by tissue culture (hollow fibre cartridges or roller cultures) for laboratory use.
Join Our Mailing List
Subscribe to our mailing list and get biology related news and updates to your email inbox.