Top : Methods and Protocols : Immunology
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Intracytoplasmic staining for cytokines
- Protocol for intracytoplasmic staining of cytokines for FACS analysis.
http://www.molbiol.ox.ac.uk/pathology/tig/cytostn.html
Added: 17-Mar-2000 Hits: 1166 Rating: 1.00 Votes: 2
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Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
- This note is not intended as a full protocol for making monoclonal antibodies, but is to give a few hints and tips for the use of Y3/Ag1.2.3. Generally speaking the methodology is similar to making murine (eg. with NS0) monoclonal antibodies, but there are a few critical differences
http://www.molbiol.ox.ac.uk/pathology/tig/y3mabs.html
Added: 16-Mar-2000 Hits: 653 Rating: 1.00 Votes: 1
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Purification of Endotoxin Free mAbs
- The following method is recommended for purification of monoclonal antibodies produced by tissue culture (hollow fibre cartridges or roller cultures) for laboratory use.
http://www.molbiol.ox.ac.uk/pathology/tig/mabpur.html
Added: 16-Mar-2000 Hits: 713 Rating: 0 Votes: 0
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