Protocol for intracytoplasmic staining of cytokines for FACS analysis.
Quick and simple method for conjugating mAbs.
This note is not intended as a full protocol for making monoclonal antibodies, but is to give a few hints and tips for the use of Y3/Ag1.2.3. Generally speaking the methodology is similar to making murine (eg. with NS0) monoclonal antibodies, but there are a few critical differences
The following method is recommended for purification of monoclonal antibodies produced by tissue culture (hollow fibre cartridges or roller cultures) for laboratory use.