Biochemistry

Biochemistry

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biochemistry resources

Biochemistry is often referred as biological chemistry and it is the study of chemical processes within and relating to living organisms. By controlling information flow through biochemical signaling and the flow of chemical energy through metabolism, biochemical processes give rise to the complexity of life. Here are some excellent resources on biochemistry topics.

Bart’s Cookbook and Lab Protocols

This page details the protocols used in the Sefton Lab at the Salk Institute. Among the techniques described are Westerns, ECL, phosphoaminoacid analysis, two-dimensional peptide mapping, CNBr mapping, V8 partial proteolytic mapping, general tissue culture methods and infection with retroviruses, biosynthetic labeling, and immunoprecipitation. The protocols include solution recipes and are filled with useful and practical hints.

Biochemical techniques

List of biochemical techniques from the Melbourne Signal Transduction Group

Western blot protocol

This protocol is a gift from Richard Huganir’s lab.

Ambros Lab Protocol Collection

A comprehensive collection of nematode-related protocols and information, this site includes general methods, equipment, microinjection, staining, dauer protocols, mutants, and drugs. This is a valuable resource for anyone working in this area.

mRNA Purification

Protocol for the purification of mRNA.

Purification of mitochondrial DNA

Purification of mitochondrial DNA: MtDNA from Protists and MtDNA from Fungi.

SDS – Polyacrylamide Gels

Protocols for the preparation of SDS – Polyacrylamide Gels.

SDS-PAGE

SDS polyacrylamide gel electrophoresis manual

SDS-PAGE

Silver Staining of SDS-PAGE gels.

Silver staining of protein gels

This protocol is adapted from Shevchenko et al., Analytical chemistry 68, p 850-858 (1996) and is especially suited for 1 mm gels.

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